Abstract
Background. Inflammation is an evolutionary developed general biological protective and adaptive reaction of the body in response to the alterative agents of allergic, toxic genesis influence. Modern treatment of inflammation requires prolonged administration of anti-inflammatory both steroid and non-steroidal pharmacological agents which increase the risk of expressive adverse reactions and complications development. We were interested in the anti-inflammatory effects of plant polyphenols, especially ellagic acid, and autologous platelet-rich plasma investigation. Aim: to determine the expression of platelet-rich plasma anti-inflammatory effect in conditions of its complex use in a model of carrageenan-induced exudative inflammation of rats paw. Materials and methods. In the work, carrageenan-induced paw edema was induced in rats in conditions of acute experiment. Platelet-rich plasma and ellagic acids separate and combined administration was used with therapeutic purposes in animals. Diclofenac sodium was chosen as a reference drug. The aseptic exidative paw edema expression - the volume and thickness of inflammatory edema, as well as the severity of somatosensory (pain) sensitivity in the tail-flick and “hot plate” tests - were determined 30 min, 1, 2, 4 and 6 hrs after carrageenan administration. Results.The data obtained indicate the achieved possibility of anti-inflammatory treatment efficacy increasing by plateletrich plasma adding to the pharmacological regimen. This effect is characterized by the severity of rats’ paw inflammatory edema reduction, an inflammatory index decreases as well as the somatosensory (pain) sensitivity restoration in animals in conditions of carrageenan-induced aseptic inflammation after platelet-rich plasma and ellagic acid combined administration. The investigated compounds anti-inflammatory effect was maximal at the delayed stages of inflammatory reaction, namely, starting from 4 hour and continued till the end of the trial. Conclusion. The expression of carrageenan-initiated aseptic inflammation suppressed as a result of platelet-rich plasma and ellagic acid combined administration due to this complex pharmacological scheme impact on the main chains of the inflammatory reaction development. The revealed platelet-rich plasma and ellagic acid combined administration antiinflammatory efficacy together with this protective effect pathogenetic background in conditions of rat paw carrageenaninitiated inflammation prove the reasonability of this pharmacological scheme anti-inflammatory efficacy clinical testing